Table 4 gives examples for quantitative drought responses of antioxidative enzymes and enzymes involved in regeneration of nonprotein. Effect of drought stress on growth parameters, osmolyte. Effects of drought stress on antioxidant enzymes in seedlings of different wheat genotypes article pdf available in pakistan journal of botany 471. Drought stress affects various physiological and metabolic processes in rice oryza sativa l. The objective of this study was to investigate the effect of micronutrients application on the antioxidant enzymes metabolism of sunflower oil under drought stress.
Finally, this research indicated that the bivanij cultivar is the most tolerant to drought stress among the studied cultivars. When rice seedlings grown for 10 and 20 days were subjected to in vitro drought stress of. Application of 20 gha selenium increased enzymes activity except the catalase. In plant cells, this occurs when the generation of reactive oxygen species ros overwhelms the antioxidant defenses. Introduction based on various observations, water stress induces oxidative stress in plants manivannanet al. Antioxidant enzymes, drought stress, grapevine, malondialdehyde, salicylic acid. Effects of coronatine on growth, gas exchange traits. Various related research indicates that the capacities and activities of antioxidant enzymes are correlated with plant resistance to abiotic stress, including drought stress 5 8 12 14. Effect of soil drought stress on leaf water status. Effect of drought stress on antioxidant enzymes activity. The changes in the antioxidant capacities such as 2,2azinobis3ethylbenzthiazoline6sulphonic acid abts and. Cerasus humilis, grown in the northern areas of china, may experience water deficit during their life cycle, which induces oxidative stress.
Drought stress is one of the most important abiotic stress factors which are generally accompanied by heat stress in dry season. Nonavailability of highyielding varieties suitable for cultivation under drought condition lead towards a sharp decline in rice yield. Effects of coronatine on growth, gas exchange traits, chlorophyll content, antioxidant enzymes and lipid peroxidation in maize zea mays l. Effect of potassium levels on antioxidant enzymes and malondialdehyde content under drought stress in sunflower helianthus annuus l.
The antioxidant defence system in the plant cell comprises both enzymatic and nonenzymatic components. During drought stress, upregulation of antioxidant systems occurs at both the transcriptional and posttranscriptional level. Pdf effect of drought stress on water status, elecrolyte. Regulation of pea cytosolic ascorbate peroxidase and other. Effect of salt and drought stress on antioxidant enzymes. Enhancement of antioxidant defense in plants can thus increase tolerance to different stress factors. Read effect of salt and drought stress on antioxidant enzymes activities and sod isoenzymes of liquorice glycyrrhiza uralensis fisch, plant growth regulation on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at. Ghanati 2 abstract drought is one of the most important abiotic stress es affecting plant growth and development. To evaluate the effects of drought stress on antioxidant enzymes activity of three chickpea cultivars, an experiment was conducted at the experimental field of the islamic azad university, karaj branch and laboratories of tarbiyat moallem. Kochia showed high tolerance against drought and salinity stresses and different antioxidant enzymes had different behavior under stress conditions.
Exposure to abiotic stresses including drought, chilling, salinity, etc. Drought stress preferentially enhanced the activities of superoxide dismutase sod and guaiacol peroxidase pod whereas it decreased catalase cat. Abstractthe comparative effects of drought stress on seed performance, growth parameters, free proline content, lipid peroxidation, and several antioxidative enzymes activities were studied in both cultivars of brassica napus l. The effects of slight, moderate and intense drought 70, 35 and 23% of the regular irrigation, respectively on growth parameters, osmolyte content, oxidative stress markers, antioxidant enzymes, glycyrrhizin biosynthesis genes and root glycyrrhizin concentration and contents, have been assessed in five iranian licorice genotypes grown in the. Effects of drought stress on the seedling growth, development. Effects of drought stress on the lipid peroxidation and. Another mechanism that could play a role in the droughtinduced inhibition of n 2 fixation, but has received much less attention, is oxidative stress. Oxidative stress in plants under drought conditions and the. Response of antioxidant enzyme activities of sugar beet to. Melatonin is an important biologically active hormone that plays a vital role in plant growth and development. The most effective level of drought stress to elevate the capabilities and enzyme activity of antioxidant as well as it gene expression was observed at 15% peg, whereas abts scavenging activity was increased 20% over the control, hydroxyl scavenging activity up to 14%, cat and apx activity increased 4 5 times over the control. Exogenous melatonin confers drought stress by promoting.
Drought tolerance is correlated with the activity of antioxidant. However, studies on biochemical factors and antioxidant enzymes during drought stress in a drought tolerant crop like horsegram still remain elusive. Effect of drought stress on the antioxidant system and redox homeostasis. The effect of micronutrients on antioxidant enzymes. The role of the plant antioxidant system in drought tolerance. Dec 17, 2019 drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in fraxinus ornus leaves. Drought stress, potassium fertilizer, antioxidant enzymes, sunflower introduction adequate water and nutrient supply are important factors affecting optimal plant growth and successful crop production. Water stress is one of the severe limitations of crop growth especially in arid and semi.
Singh division of biochemistry, iari, new delhi110012, india corresponding author a b s t r a c t peroxidase enzymatic activities measured. Growth performance and antioxidant enzyme activities of. The effect of drought stress and recovery from drought on the relative abundance of. Effect of drought on pigments, osmotic adjustment and.
As past research has shown, environmental drought stress has caused oxidative damage in plants larson, 1995. The study was undertaken to identify the responses of antioxidant enzyme activities and their isozyme patterns in seedlings of 10 oilseed rape brassica napus l. Effects of drought stress on the lipid peroxidation and antioxidant enzyme activities in two canola brassica napus l. Drought stress increased mean germination rate and mean of day germination in both cultivars, and its effect was more pronounced in rgs003. Potential of antioxidant enzymes in depicting drought. For field studies, a spiltsplit plot design on the basis of rcbd with four replications was used. Morphological, physiochemical and antioxidant responses of. Further, very little is known, about the link between drought stress, lox activity, antioxidant enzymes, pro and mda levels, response in the millets. Exogenous melatonin confers drought stress by promoting plant. Antioxidant enzymes to mitigate drought stress in rice genotype m. Although, this crop is adapted to resist severe drought 17, little is known about its mechanisms of osmotic adjustment and ability to repair the damage caused by drought induced oxidative stress.
To overcome oxidative damage under drought stress conditions, plants must have efficient antioxidant system stepien and klobus, 2005. To evaluate the effects of drought stress on antioxidant enzymes activity of three chickpea cultivars, an experiment was conducted at the experimental field of the islamic azad university, karaj branch and laboratories of tarbiyat moallem university of tehran. A correlation analysis showed that antioxidant enzyme activities are inversely correlated to the expression levels of the ppatgs. Regulation of pea cytosolic ascorbate peroxidase and other antioxidant enzymes during the progression of drought stress and following recovery from drought. Influence of drought stress on assimilation of carbon, activity of photosynthetic enzymes, heat shock proteins, antioxidants. Drought tolerance is correlated with the activity of. Antioxidant enzyme responses and crop yield of wheat under. The activities of superoxide dismutase sod and peroxidase pod and contents of endogenous aba in plants were measured at 3 days of drought stress in cv. Pdf antioxidant enzyme changes in response to drought. Some antioxidant enzymes such as sod, cat, apx, and gpx are better studied figure 3 and 4, but in general all enzymes have potential defense antioxidant activity helping in scavenging ros in different ways, either by dismutation of o 2 to h 2 o 2, reduction of h 2 o 2, maintenance of the asa pool, or of the adequate levels of gsh and gssg.
The effect of drought stress and abscisic acid aba applied at tillering stage 55 days after sowing was compared in 2 wheat cultivars differing in drought tolerance. Drought has been shown to increase the activity of antioxidant enzymes such as catalase cat, ascorbate peroxidase apx and guaiacol peroxidase gpx 7,10,11. Drought effects are a high risk to rice production, especially in parts of peninsular malaysia. Reactive oxygen species and antioxidant enzymes involved in. Effect of potassium levels on antioxidant enzymes and. Effect of salicylic acid application on oxidative damage.
The results of this study suggested that the amount of antioxidant enzymes determine drought stress tolerance in wheat. These multifarious modulations in plants were related to stress. Impact of drought stress during germination on antioxidant. Antioxidant enzymes regulated in plants under drought. Under drought stress, the photosynthetic abilities of peach leaves decreased, while antioxidant enzyme activities, autophagy and atg expression increased. Oxidative stress in five wheat varieties triticum aestivum l. The role of the plant antioxidant system in drought tolerance mdpi. Additionally the activity of antioxidant enzymes sod, apx, dhar and gr resulted affected by drought stress. There exist multiple and often redundant stress sensors, which transduce the stress signal through secondary signalling. Sep 29, 2006 the effects of salinity and drought on the antioxidative system sod, pod, cat were studied in liquorice seedlings glycyrrhiza uralensis fisch. Effect of soil drought stress on leaf water status, membrane. Effect of salicylic acid application on oxidative damage and. Role of abscisic acid and drought stress on the activities of.
Leaf is one of the early sensors for the drought stress and is important to study drought tolerance mechanism. Oxidative stress in plants under drought conditions and the role of different enzymes leonora mansur mattos and celso luiz moretti brazilian agricultural research corporation, brazil e n z y m e e ngi n e e r i n issn. Microbial inoculation in drought challenged rice triggered multipronged steps at enzymatic, nonenzymatic and gene expression level. Drought induces oxidative stress and enhances the activities. Pdf water stress, antioxidant enzyme activity and lipid. The response of carbon metabolism and antioxidant defenses. Pdf effects of drought stress on antioxidant enzymes in. Article pdf available april 2020 with 70 reads how we measure reads. Pdf effect of drought stress on antioxidant enzymes. Drought stress has contrasting effects on antioxidant enzymes activity and phenylpropanoid biosynthesis in fraxinus ornus leaves. It is the major yieldlimiting factor of crop plants and it actively and continuously determines the natural distribution of plant species.
In particular, it has been investigated for its roles in abiotic stress management. Plants cope with drought stress by manipulating key physiological processes like photosynthesis, respiration, water relations, antioxidant and hormonal metabolism. All these responses increase scavenging capacity against ros. Antioxidant enzyme changes in response to drought stress in ten. The effect of drought stress and recovery on the relative abundance of antioxidant transcripts. The response of carbon metabolism and antioxidant defenses of. Amounts of malondialdehyde mda and hydrogen peroxide h2o2 remained unchanged in sesupplemented waterdeficit plants obviously because of an efficient scavenging following significant enhancement of catalase cat and glutathione peroxidase gshpx activities. The activities of sod and pod were upregulated by salt and drought stress, while cat activity decreased.
Stage specific upregulation of antioxidant defence system in. The antioxidant system including antioxidant enzymes such as superoxide dismutase sod, peroxidase pod, and catalase cat as. Exogenous melatonin confers drought stress by promoting plant growth, photosynthetic capacity and antioxidant defense system of maize seedlings shakeel ahmad 1, 2, muhammad kamran 1, 2, 3, ruixia ding 1, 2, xiangping meng 1, 2, haiqi wang 1, 2, irshad ahmad 1, 2, shah fahad 4, qingfang han 1, 2. Comparison of these responses will help identify drought tolerance mechanisms in the melon cultivars k. To meet the needs of the growing world population, it is essential to effectively utilize dehydrated soil in droughtprone areas. The results of biochemical analysis showed the activity of antioxidant enzymes increases under stress conditions. Drought stressinduced oxidative stress and antioxidative. We hypothesized that the two shrubs had higher capacities of osmotic adjustment and antioxidant defense than the four trees, which permitted their higher drought tolerance and better photosynthetic performance under drought stress.
Microbial inoculation in rice regulates antioxidative. Role of abscisic acid and drought stress on the activities. Antioxidant enzymes activities pox, ppo, sod, cat and apx were significantly increased accompanied by significant decreases in nr due to drought stress. The effect of various polyethylene glycol peg concentrations to induced drought stress on the antioxidant capacities and antioxidant enzyme activities was investigated on madura local maize during germination. Effect of drought stress on antioxidant enzymes activity of. Influence of drought stress on assimilation of carbon. Indeed, drought induces oxidative stress in pea nodules gogorcena et al. Abiotic stresses, such as drought, soil salinity and extreme temperatures adversely affect the productivity and quality of economically important. Drought stress is considered as the main factor of yield limitations in arid and semiarid areas, where drought and salinity stresses are usually combined. Pdf in arid and semi arid zones, soil moisture deficit as a major adverse factor can lower leaf water potential, leading to reduced turgor and some. Drought stressinduced oxidative stress and antioxidative responses in four wheat triticum aestivum l. Response of antioxidant enzymes to drought 399 figure 2. The current experiment was carried out to investigate the protective role of melatonin in photosynthetic traits and the antioxidant defense system of maize seedling under drought stress.
Drought is one of the most important manifestations of abiotic stress in plants. The scavenging system in drought tolerant variety introduction drought is one of the major abiotic stresses. The effects of salinity and drought on the antioxidative system sod, pod, cat were studied in liquorice seedlings glycyrrhiza uralensis fisch. Activity of photosynthetic enzymes the data on water stress induced regulation of the.
Material and method this experiment was carried out at the golmakan agriculture research station iran in. Drought stress disturbs water relations, causing a reduction in wateruse efficiency. Oxidative stress in plants under drought conditions and. Plant treatments are designated as in figure 2 and statistical analysis is as described in figure 4. Effect of drought stress on oxidative damage and antioxidant. Antioxidant enzymes, wheat, seedling growth, drought, triticum aestivum l. Role of antioxidant enzymes in the ros scavenging mechanism. Induce mutation is an essential auxiliary approach to counterpart conventional breeding to produce stress tolerance rice variety. Drought stress at 75% fc and 50% fc decreased seed yieldplant, oil and carbohydrate, total phenolic content, tannins, flavonoids and antioxidant activity accompanied by significant. American journal of agricultural and biological sciences. Physiological role of glycinebetaine in alleviating the. The results showed that both salt and drought stresses could induce oxidative stress, as indicated by the increase level of lipid peroxidation. However, the responses of the antioxidant defense system in plant species to drought stress. Steadystate levels of mrnas encoding antioxidant enzymes in the bacteroids of alfalfa plants exposed to drought stress and following recovery from drought.
Seed samples were treated under 5%, 10%, and 15% of peg for 6 days. Accumulation of proline that synthesized from lglutamic acid with two enzymes of pyrroline5carboxylate p5c causes a mechanism that may store carbon and nitrogen. Impact of seed priming on proline content and antioxidant. Drought is perceived as the most significant environmental stress in agriculture worldwide, and improving yield under drought is therefore a major goal of plant breeding. Reactive oxygen species and antioxidant enzymes involved. Drought has been shown to increase the activity of antioxidant enzymes such as catalase cat, ascorbate peroxidase apx and. Although, this crop is adapted to resist severe drought 17, little is known about its mechanisms of osmotic adjustment and ability to repair the damage caused by droughtinduced oxidative stress. Therefore, the present study was framed to compare the biochemical and antioxidative responses in leaf and root tissue of a drought sensitive and a drought tolerant variety of horsegram under.
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